Selection of f4, f5 and f6 soybean breeding lines was conducted in the rice field of. Improvement of soybean meal through identification of high. Most promising one can be used in further breeding programme. Variability was also found in the seed size 100 seed weight, plant height, and number of pods figure 24. The objective of this the selection of yield fehr, 1987. Transgressive mineral accumulation values superior to that of the best parental line were observed in f2 individual plants for all minerals examined, except for na in the cross it97k10423 x brs tapaihum table 2. Transgressive segregation of isoflavone contents under the control of four qtls in a cross between distantly related soybean varieties article pdf available in breeding science 603. Introduction to segregation analysis in the mid 1800s, gregor mendel demonstrated the existence of genes based on the regular occurrence of certain characteristic ratios of dichotomous characters or traits among the o spring of crosses between parents of various characteristics and lineages. Delineating soybean maturity groups across the united states. These qtl were directly related to the appearance of transgressive individuals in the f2 for those traits which showed transgressive segregration. Two soybean cultivars, a3935 and s4240, were planted using row widths of 30, 50, and 70 cm, and twin row 50 25 50 cm in 2004 and 2005. Transgressive segregation was observed in all traits except for 100 seed weight. The recombinants generated in the crosses either recombine the good traits of two parents or superior to either of the parents used for hybridization.
One source of enhanced resistance is that generated through transgressive segregation. Based on experimental data of the soybean yield and quality from 30 sampling points, a quantitative structureactivity relationship model 2dqsar was established using the soil quality elements, ph, organic matter content and cation exchange capacity as independent variables and soybean yield or quality as the dependent variable, with spss software. Virtually all major genes currently used by animal breeders were first detected by noting strong familial trends during inspection of recorded data. Transgressive segregation introduction novel sources and enhanced levels of pathogen resistance are desirable for genetic improvement of crop plants. Construction of highdensity genetic map and qtl mapping. However, most such methods are based on mutants and usually only one gene controlling the mutant phenotype is identified. Agrobacterium soybean 1 updated 032210 agrobacteriummediated transformation of soybean and recovery of transgenic soybean plants. Hybrid offspring generally possess traits or characteristics seen in ancestral species, which are expected to be subdued or diluted when. Qtl analysis of transgressive nematode resistance in. Several reports are available on transgressive segregation for yieldrelated traits from crosses of. Ag phd information for agriculture new soybean genetics. The appearance of these trangressive extreme phenotypes can be.
On what plant traits is transgressive segregation usually. This is another application in which segregation analysis plays a big role. Part of theagricultural science commons,agriculture commons, and theplant biology commons. Transgressive segregation was observed for eight of the traits in a large. Mapping and comparison of quantitative trait loci for oleic. Improvement of soybean meal through identification of high protein qtl in lines with exotic pedigree materials and methods plant materials. Quantitative trait loci in two soybean recombinant inbred. The presence of transgressive segregants in backcross generations have also been reported for different characters in tomato franklin, 1987, grain sorghum rao et al. We examined transgressive phenotypes of flowering time in rice, and revealed transgressive segregation in f2 populations derived from a cross between parents with similar proximal days to. Genetic gain for soybean seed protein, oil, and yield in a. Transgressive segregation is the phenomenon in which segregating hybrids exhibit pheno. F 2 populations were subjected to quantitative trait locus qtl analysis for days to flowering df and maturity dm. Transgressive segregation an overview sciencedirect topics.
Only three of 1 studies failed to report extreme phenotypes for at least one character, and of 579 traits examined. In particular, the paper examines the different gender roles in soybean production, the levels and factors influencing. Compositional analyses were used to evaluate any changes in the levels of key nutrients and antinutrients in das684164 soybean which was sprayed with either 2,4d, glufosinate, both 2,4d and glufosinate, or which was not sprayed with either herbicide. Qtl analysis of transgressive segregation in an interspecific. Integration one of the most significant issues which the united states has dealt with for decades is the issue of racial segregation. Upon seed delivery, elevators do not typically analyze soybean seed for quality. The recombinant inbred lines rils developed from these two parents showed a transgressive segregation for root morphology figure 2.
This suggests that kamaishi17 possesses the same genotype e3e3e4e4 at the e3 and e4 loci as is the case in miharudaizu. Mapping quantitative trait loci controlling soybean seed starch content in an interspecific cross of williams 82 glycine max and pi 366121 glycine soja sanjeev k. United states agriculture of the national sclerotinia. Genomewide identification of quantitative trait loci for important. Crop science abstract transgressive segregation for. Genetic properties responsible for the transgressive segregation. Biplot analysis, multiple traits, transgressive segregation, genetic value, mungbean.
Identification of ssr markers linked to activity of. The effects of row spacing on yield and yield components of. We examined transgressive phenotypes of flowering time in rice, and revealed transgressive segregation in f2 populations derived from a cross between parents with similar proximal days to heading dth. Kunihiko komatsu1, shiori okuda1, masakazu takahashi1, ryoichi matsunaga2 and yoshinori nakazawa1 1national agriculture and food research organization, national agricultural research center for kyushu okinawa region, koshi, kumamoto, japan. Although soybean is classified as a moderately salttolerant crop, the final yield of soybean will be reduced when soil salinity exceeds 5 ds m1 ashraf and wu, 1994. More number of transgressive segregants was found for number of seeds per plant followed by number of pods per plant and yield per plant.
Product differentiation and segregation in agricultural. However, the same types of qtl with allelic effects opposite to those predicted by the parents were also observed for traits that did not display transgressive segregation in the f2. Transgressive segregation for oleic acid content was observed for the lines that. Analysis of quantitative trait loci for main plant traits in. Seed isoflavone profiling of 1168 soybean accessions from. Soybean is an important cash crop and a major cause of its productivity loss is abiotic stress. Pdf the success of selection in selfpollinated crops depends on the extent to which breeders can fix transgressive. An important determinant of the nutritional value and the oxidative stability of soybean oil is the oleic acid content. Transgressive segregation was observed only for seed length based on combined data. Therefore, this research was aimed to determine ssr markers for lox activity in the. Transgressive segregation is a commonly used and a correct term and acordingly such progenies obtained are known as transgressive segregants.
A parental difference would be a necessary assumption of a singleqtl model, since transgressive segregation requires at least two qtl. Cross kalitur pk 317 showing equal frequency 17 % of transgressive segregation in both generations indicated that the involvement of. Time to flowering and maturity in soybean is controlled by loci e1 to e5, and e7 to e9. Genetic markers are useful tool for estimating genetic diversity between parents and predicting the performance of traits in progeny. Canopy wilting was rated in three environments using a rating scale of 0 no wilting to 100 severe wilting and plant death. Transgressive segregation produces hybrid progeny phenotypes that exceed the parental phenotypes. Das684164 soybean with the composition of a nontransgenic control. Genetic properties responsible for the transgressive. Cloning and function analysis of a plasma membrane intrinsic. Transgressive segregation in the rils was shown for eight traits, suggesting that alleles with positive effects on the measured traits are distributed among the parents. Then, qtl mapping and an effect analysis were conducted for 5 main plant traits i. If the parents are genetically distant and differ for more genes that affect the trait, the probability of obtaining such genotypes increases.
Quantitative trait locus mapping of soybean maturity gene e5. The objective of this paper is to assess the real impact of soybean on the lives of smallscale farmers in benue state, nigeria. Both f 2 plants and f 3 families were identified that were either homozygous or heterozygous for loci controlling palmitic acid. Quantitative trait loci mapping of pubescence density and flowering time of insectresistant soybean glycine maxl. Ssr markers linking to seed traits and total oil content. Integration of lodging resistance qtl in soybean scientific reports. Qtls conditioning early growth in a soybean population. Grain dimensions are known to be quantitative traits and to be. The diverse genotypes identified here will be helpful in planning crosses and achieving transgressive segregation for soybean isoflavone improvement. Relationship between soybean yieldquality and soil quality. Unlike heterosis, extreme phenotypes caused by transgressive segregation are heritably stable.
May 31, 2017 nextgeneration sequencing ngsbased bulkedsegregant analysis bsa approaches have been proven successful for rapidly mapping genes in plant species. These transgressive segregants were successfully exploited in developing cultivars possessing combinations of various selected traits. Both usually produce fertile hybrids, and there is no marked obstacle to gene exchange, although the sterility due to abnormal chromosome pairing has often been observed. Mutant alleles of fad21a and fad21b combine to produce. Soybean is an economic crop used as a main source of vegetable oil. The appearance of these trangressive extreme phenotypes can be either positive or negative in terms of fitness.
As in the first two editions, this edition describes the most recent advances in soybean science and technology and the trends in soybean usage that are changing the way we live. Elevation of the oleate content levels leads to the improvement of soybean oil quality. As seen with the stb in kenya and riesebergs sunflowers, transgressive segregation can be used to create a species that is more adaptable and resistant in areas where there is environmental stress. Jan 26, 2015 law of segregation monohybrid cross a cross between 2 individuals involving 1 character allele pairs separate and each goes into a different gamete. In our model, transgressive segregation through hybridization, unlike mutation accumulation, enabled instantaneous and repeated formation of potentially adaptive novel phenotypes through assembling genetic materials from parental lineages into novel genotypes, thereby promoting ecological speciation and adaptive radiation. Hamidreza dargahi, 1 patcharin tanya, 2 prakit somta, 2 jun abe, 3 and peerasak srinives, 2. Transgressive segregation, adaptation and speciation citeseerx. Mapping and introgression of qtl involved in fruit shape. The discussion was closed on 17 november 2009 with a consensus to merge. The nonmendelian inheritance of a transgene has been recorded with a frequency between 10% and 50% in transgenic plants produced either by agrobacteriummediated transformation or through particle bombardment. Transgressive segregation indicates that the qtl from n0610035 and n0610011 parents are different than those from the. Dna tests and segregation analysis for genetic disorders. To submit an update or takedown request for this paper, please submit an updatecorrectionremoval request.
This indicated the presence of transgressive segregation on the breeding materials. Mapping quantitative trait loci for tolerance to pythium. Transgressive segregation for oleic acid content was observed for the. Transgressive mineral accumulation values superior to that of the best parental line were observed in f 2 individual plants for all minerals examined, except for na in the cross it97k10423 x brs tapaihum table 2. The soybean research is indexed in soybean abstract of cab international, uk and indian science abstracts of niscair, india financial assistance received from the indian council of agricultural research, the society for soybean research and development thankfully acknowledges the new delhi for printing of the journal. The quality characteristics of soybean meal sbm can ultimately. Pdf transgressive segregation analysis of multiple traits in. In this study, the genetic bases for grain characteristics were examined by comparing two accessions representing the two rice subspecies by qtl analysis. Assessment of variability and identification of transgressive. Conventional soybean breeding and selection practices. Construction of highdensity genetic map and qtl mapping of yieldrelated and two quality traits in soybean rils population by radsequencing.
Transgressive segregation appears to be ubiquitous in plant hybrids. Article pdf available in theoretical and applied genetics 1286 march 2015 with 465 reads. Transgressive segregation of isoflavone contents under the control of four qtls in a cross between distantly related soybean varieties. Physiological basis for transgressive yielding in beanmaize intercrops ethel ann clark iowa state university follow this and additional works at. For the contribution history and old versions of the redirected article. Nongenetically modified and specialty corn and soybean crops in iowa introduction providing consumers with choices through the use of specialty products has the potential to enhance welfare, as long as identity is preserved in food and feed products for.
Appearance of such superior recombinants in the segregating generations is referred to as transgressive segregation. Transgressive phenotype was nominated for deletion. Selection of f4, f5 and f6 soybean breeding lines for high. Mapping quantitative trait loci for yieldrelated traits in soybean glycine max l. A track on these transgressive segregants should be maintained and forwarded to further generation till they reach nearly homozygous condition. Crosses were made between wheat varieties joss cambier, nord desprez and maris bilbo, all classified as susceptible to yellow rust in field tests, and between cappelle desprez and maris huntsman, both classified as moderately and durably resistant.
Ssr markers linking to seed traits and total oil content in soybean j. Quantitative trait loci mapping of pubescence density and. View uniform field trial data here soybean parentage information. Agrobacteriummediated transformation of soybean and recovery. Transgressive segregation of isoflavone contents under the. Hybridization can promote adaptive radiation by means of. Transgressive segregation it is the formation of extreme phenotypes, or transgressive phenotypes, observed in segregated hybrid populations compared to phenotypes observed in the parental lines. Transgressive segregation was observed for the ril popu. A transgressive segregation factor rkn2 in gossypium. Genetic diversity and its use in soybean abe jun hokkaido university introduction cultivated and wild soybeans form a primary gene pool hymowitz, 2004. Transgressive segregation was observed for all traits in both years. Selection for resistance to yellow rust among the progeny was carried out using races of puccinia striiformis able to overcome all the known race. Detection of quantitative trait loci for seed size traits.
The genetic architecture necessary for transgressive. Mapping quantitative trait loci for yieldrelated traits. Hybrid offspring generally possess traits or characteristics seen in ancestral species, which are expected to be. Its contents were merged into transgressive segregation. Transgressive segregation for resistance to yellow rust in. Pdf transgressive segregation of isoflavone contents under. In a postcivil rights era, there is a common tendency to assume that racism is no longer a pressing social concern in america due to the gradual erosion of whiteness. The selection would be effective when there was wide. Frontiers nextgeneration sequencing from bulkedsegregant.
If i switch to 100% libertylink or 100% xtend this year, will they yield and have a good defensive package. In genetics, transgressive segregation is the formation of extreme phenotypes, or transgressive phenotypes, observed in segregated hybrid populations compared to phenotypes observed in the parental lines. Mapping quantitative trait loci controlling soybean seed. Transgressive phenotypes can be products of epistatic interactions or additive effects of multiple recombined loci. Combined over environments, tokyo averaged 41 and 17% taller plants than s100 at the v7 and v10 stages of development. With all the new things coming in soybeans, it has been really fun putting together the ag phd field day and also doing large and small scale research with new soybean traits and genetics on our farm and across the country. Genetic mapping of qtls conditioning soybean sprout yield. The production of extreme or transgressive phenotypes in segregating hybrid populations has been speculated to contribute to niche divergence of hybrid lineages.
Merrill is widespread, with applications in food, fuel, livestock feed, and industrial products. The broad sense heritability on a 2year basis varied from 0. Transgressive segregation creates an opportunity for new hybrid species to arise that are more fit than their ancestors. Through qtl analysis the transgressive segregation for rkn resistance was characterized further in six other genetic populations derived from three parental lines, g. However, there is no reports about qtl of this trait in soybean cultivars of thailand. Transgressive segregation was observed for both ril populations in this study, suggesting that the combination of genetic components from both parents could strengthen the tolerance to disease rieseberg et al. Three polymorphisms found in the fad21b alleles of two soybean. Improved cotyledonary node method using an alternative explant derived from mature seed for efficient.
Given the potentially large confounding influence of environment, polygenes, and segregation at the major. In pop1, qrrw20 was detected from e05226t, but no qtl was detected from e09014. Aurora daz1, belkacem zarouri2, mohamed fergany3, iban eduardo3, jose. Genetic control and transgressive segregation of zinc. Transgressive segregation, adaptation and speciation heredity. Here, we assess the frequency of transgressive segregation in hybrid populations, describe its genetic basis and discuss the factors that best predict its occurrence. Changes in genome content generated via segregation of non. Genetic markers in estimating similarity of soybean. Transgressive hybrids as hopeful monsters springerlink. Note that transgressive segregation is a phenomenon specific to segregating hybrid generations and refers to the fraction of individuals that exceed parental phenotypic values in either a negative or positive direction.
Transgressive segregation was found to be more frequent in plants than in animals. Pdf transgressive segregation of isoflavone contents. Variation and transgressive segregation in the backcross. In this study, ngsbased bsa was employed to map simultaneously two qualitative genes controlling cotyledon color of. A valuable tool for the functional study of genes and the production of agronomically improved plants milena schenkel homrich1, beatriz wiebkestrohm1,2, ricardo luis mayer weber1.
For example, soybeans value delineating soybean maturity groups across the united states spyridon mourtzinis and shawn p. Transgressive segregation was observed in all traits, revealing that. The transgressive segregation of sprout yield, as well as the existence of two qtls conditioning greater than 10% of the phenotypic variation in sprout yields provides an opportunity to select for progeny lines with a greater sprout yield than currently preferred cultivars such as pureunkong. Polygenic inheritance of canopy wilting in soybean glycine. When we get progenies having observation for a trait. The apparent lack of transgressive segregation in the f 2 generation was unexpected. As nouns the difference between aggregation and segregation is that aggregation is the act of collecting together aggregating while segregation is the setting apart or separation of things or people, as a natural process, a manner of organizing people that may be voluntary or enforced by law. Transgressive segregation, adaptation and speciation. This is caused in part by heterosis, which is most pronounced in firstgeneration hybrids. Transgressive segregation, heritability, and number of genes controlling durable resistance to stripe rust in one chinese and two italian wheat cultivars. Genetic variants in root architecturerelated genes in a. This study was conducted to map the e5 locus using f 2 populations expected to segregate for e5.
The soybean parentage information in this database was partially gleaned from the uniform soybean tests for the southern and northern regions as well as other sources such as usda technical bulletins, variety registrations and pvp applications. We compare several epistatic and additive models of transgressive segregation in hybrids and find that they are special cases of a general, classic quantitative genetic model. The broadsense heritability on a 2year basis varied from 0. It has been theoretically proposed that multiple linked quantitative trait loci qtls play a role in the accumulation of hidden variation within and between populations. Physiological basis for transgressive yielding in beanmaize.
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